DESCRIPTION OF THE INVENTION

In one aspect of the present invention oil-in-water emulsion formulations (EW) are provided comprising

a) one or more pesticidal active ingredients selected among avermectins

b) one or more organic solvents selected among phthalates

c) an emulsifier system comprising one or more surfactants

d) water

The formulations according to the invention provide a significant stabilization of the active ingredients compared to oil-in-water formulations comprising avermectins according to the prior art and maintain the benefits of oil-in-water emulsions. Further, the formulations significantly reduce the degradation of the avermectin(s) also when exposed to light.

As such the present invention provides a method for stabilising avermectins in oil-in-water emulsion formulations using the above composition. Preferably the formulations provide stabilisation of the avermectin(s) to an extent that less than 5%, more preferably 3%, of the initial concentration of the avermectin(s) has degraded when the formulations are stored at 54° C. for 14 days; or less than 10%, more preferably 5%, of the initial concentration of the avermectin(s) has degraded when the formulations are stored at 70° C. for 14 days.

The term oil-in-water emulsion formulation means the undiluted formulation.

The avermectin(s) is e.g. selected among Abamectin, Aversectin C, Doramectin, Emamectin (optionally in the form of its benzoate salt), Eprinomectin, Ivermectin and Selamectin and especially selected among Abamectin, Aversectin C, Ivermectin and Emamectin (optionally in the form of its benzoate salt) with Abamectin being the most preferred choice.

For the purpose of this invention, all percentages expressed herein are percentage by weight, unless otherwise specified.

The concentration of the avermectin is generally between 0.001 to 30%, preferably 0.1 to 10%, and more preferably 1 to 5%.

The phthalate(s) used as organic solvent is chosen among dialkyl or alkyl aryl esters of 1,2-benzenedicarboxylic acids (it being understood that the alkyl or alkyl aryl groups may be the same or different and the alkyl groups linear or branched) and include diethylhexyl phthalate, ethylhexyl phthalate, dimethyl phthalate, diethyl phthalate, butylbenzyl phthalate, dibutyl phthalate, diisononyl phthalate, and dioctyl phthalate. Preferred are dimethyl phthalate, diethyl phthalate and diisononyl phthalate and especially diethyl phthalate.

The amount of phthalate is generally between 10 to 60%, preferably 20-50%. The emulsifier system comprising one or more surfactants is chosen among anionic, cationic, nonionic, zwitterionic and polymer surfactants or mixtures thereof.

Examples of suitable anionic surfactants include alkali, alkaline earth or ammonium salts of the fatty acids, such as potassium stearate, alkyl sulfates, alkyl ether sulfates, alkylsulfonates or iso-alkylsulfonates, alkylbenzenesulfonates such as sodium dodecylbenzenelsulfonate, alkylnaphthalenesulfonates, alkyl methyl ester sulfonates, acyl glutamates, alkylsulfosuccinates, sarcosinates such as sodium lauroyl sarcosinate, taurates or ethoxylated and phosphorylated styryl-substituted phenols. Examples of suitable cationic surfactants include halides or alkyltrimethylammonium alkyl sulfates, alkylpyridinium halides or dialkyldimethylammonium halides or dialkyldimethylammonium alkyl sulfates. Examples of suitable nonionic surfactants include alkoxylated animal or vegetable fats and oils such as corn oil ethoxylates, castor oil ethoxylates, talo fat ethoxylates, glycerol esters such as glycerol monostearate, fatty alcohol alkoxylates and oxoalcohol alkoxylates, fatty acid alkoxylates such as oleic acid ethoxylates, alkylphenol alkoxylates such as isononylphenol ethoxylates, fatty amine alkoxylates, fatty acid amide alkoxylates, sugar surfactants such as sorbitan fatty acid esters (sorbitan monooleate, sorbitan tristearate), polyoxyethylene sorbitan fatty acid esters, alkyl polyglycosides, ethoxylated styryl-substituted phenols, N-alkylgluconamides, alkylmethyl sulfoxides, alkyldimethylphosphine oxides such as tetradecyldimethylphosphine oxide.

Examples of suitable zwitterionic surfactants include alkylbetaines, alkylamidobetaines, amino-propionates, aminoglycinates, imidazolinium betaines and sulfobetaines.

Examples of polymer surfactants include di-, tri- or multi-block polymers of the (AB)x, ABA and BAB type, such as polyethylene oxide block polypropylene oxide, polystyrene block polyethylene oxide, AB comb polymers such as polymethacrylate comb polyethylene oxide or polyacrylate comb polyethylene oxide.

The surfactants mentioned are all known compounds.

The amount of surfactant in the formulations is generally between 0.1-20%, preferably between 0.5-15% and more preferably between 1-10%.

It is preferred to use as emulsifier system, one or more surfactants selected among anionic surfactants, more preferably ethoxylated and phosphorylated styryl-substituted phenols and alkyl ether sulfates.

Further optionally auxiliaries which may be included in either the organic or aqueous phase (depending on solubility) include co-solvents, pH-adjusters, thickeners, film-forming agents, antifreeze agents, preservatives, antifoaming and defoamer agents, spreading agents, stickers, wetting agents, structuring agents, stabilisers, UV-protectants and one or more additional insecticides. Such auxiliaries are generally known within the art of formulation chemistry, and although a specific ingredient is classified as falling within one category, it may well serve the purpose of any of the others.

Suitable co-solvents include mineral oils and vegetable oils, e.g. avocado oil, coconut oil, rape seed oil, maize oil, sesame oil, olive oil, soybean oil, palm oil, grape seed oil, almond oil, linseed oil, peanut oil, walnut oil, tall oil, thistle seed oil, wheat germ oil, sunflower oil, poppy-seed oil, cottonseed oil, persic oil, apricot oil, jojoba oil, castor oil and sesame oil.

The pH adjusters include both acids and bases of the organic or inorganic type. Suitable pH adjusters include organic acids and alkali metal compounds. The organic acids include those such as citric, malic, adipic, cinnamic, fumaric, maleic, succinic, and tartaric acid, and the mono-, di-, or tribasic salts of these acids are suitable organic acid salts. Suitable salts of these acids are the soluble or meltable salts and include those salts in which one or more acidic protons are replaced with a cation such as sodium, potassium, calcium, magnesium, and ammonium. Alkali metal compounds include hydroxides of alkali metals such as sodium hydroxide and potassium hydroxide, carbonates of alkali metals such as sodium carbonate and potassium carbonate, hydrogencarbonates of alkali metals such as sodium hydrogencarbonate and alkali metal phosphates such as sodium phosphate.

The amount of addition of pH adjuster to the composition is at one's option, but it has been found that the pH value of the emulsions, i.e. prior to dilution in for example spraying equipment, to some extent have an influence on the stability of the avermectin, and the optional pH adjusters are suitably present in amounts to ensure a pH-value of the emulsions from 3 to 10, preferably 4-9 and even more preferably 5-8. However, one need not necessarily add pH adjusters as the emulsifier system by itself or the optionally auxiliaries, depending on choice of components, may ensure that the pH-value is within the preferred range.

Thickeners and film-forming agents include starches, gums, casein and gelatine, polyvinyl pyrrolidones, polyethylene and polypropylene glycols, polyacrylates, polyacrylamides, polyethyleneimines, polyvinyl alcohols, polyvinyl acetates, and methyl-, hydroxyethyl- and hydroxypropylcelluloses and derivatives thereof.

Examples of the antifreezing agent include ethylene glycol, diethylene glycol, propylene glycol and the like.

Typical preservatives include methyl and propyl parahydroxybenzoate, 2-bromo-2-nitro-propane-1,3-diol, sodium benzoate, formaldehyde, glutaraldehyde, O-phenylphenol, benzisothiazolinones, 5-chloro-2-methyl-4-isothiazolin-3-one, pentachlorophenol, 2-4-dichlorobenzylalcohol and sorbic acid and derivatives thereof.

Preferred anti-foaming and defoamer agents are silicone based compounds e.g. polyalkylsiloxanes.

The optional additional insecticide (including acaricides and nematicides) can advantageously be included for example to widen the spectrum of action or to prevent the build-up of resistance. Suitable examples of such additional insecticides are e.g.:

acephate, acetamiprid, acrinathrin, alanycarb, aldicarb, alphamethrin, amitraz, azadirachtin, azinphos, azocyclotin, Bacillus thuringiensis, bendiocarb, benfuracarb, bensultap, betacyfluthrin, bifenazate, bifenthrin, bistrifluoron, BPMC, brofenprox, bromophos, bufencarb, buprofezin, butocarboxin, butylpyridaben, cadusafos, carbaryl, carbofuran, carbophenothion, carbosulfan, cartap, chloethocarb, chloroethoxyfos, chlorfenapyr, chlorofenvinphos, chlorofluazuron, chloromephos, chlorpyrifos, chromafenozide, cis-resmethrin, clothianidin, clocythrin, clofentezine, cyanophos, cycloprothrin, cyfluthrin, cyhalothrin, cyhexatin, cypermethrin, cyromazine, deltamethrin, demeton, difenthiuron, diazinon, dichlofenthion, dichlorvos, dicliphos, dicrotophos, diethion, diflubenzuron, dimethoate, dimethylvinphos, dinotefuran, dioxathion, disulfoton, edifenphos, esfenvalerate, ethiofencarb, ethion, ethofenprox, ethoprophos, etoxazole, etrimphos, fenamiphos, fenzaquin, fenbutatin oxide, fenitrothion, fenobucarb, fenothiocarb, fenoxycarb, fenpropathrin, fenpyrad, fenpyroximate, fenthion, fenvalerate, fipronil, flonicamid, fluazinam, fluazuron, flucycloxuron, flucythrinate, flufenoxuron, flufenprox, fluvalinate, fonophos, formothion, fosthiazate, fubfenprox, furathiocarb, gamma-cyhalothrin, HCH, heptenophos, hexaflumuron, hexythiazox, imidacloprid, indoxacarb, iprobenfos, isazophos, isofenphos, isoprocarb, isoxathion, lambda-cyhalothrin, lufenuron, malathion, mecarbam, mevinphos, mesulfenphos, metaldehyde, methacrifos, methamidophos, methidathion, methiocarb, methomyl, methoxyfenozide, metolcarb, milbemectin, monocrotophos, moxidectin, naled, nitenpyram, omethoate, oxamyl, oxydemethon M, oxydeprofos, parathion A, parathion M, permethrin, phenthoate, phorate, phosalone, phosmet, phosphamidon, phoxim, pirimicarb, pirimiphos, profenofos, promecarb, propaphos, propoxur, prothiofos, prothoate, pymetrozin, pyrachlophos, pyridaphenthion, pyresmethrin, pyrethrum, pyridaben, pyrimidifen, pyriproxifen, quinalphos, salithion, sebufos, silafluofen, spinosad, spirodiclofen, sulfotep, sulprofos, tebufenozid, tebufenpyrad, tebupirimiphos, teflubenzuron, tefluthrin, temephos, terbam, terbufos, tetrachlorvinphos, thiacloprid, thiafenox, thiamethoxam thiodicarb, thiofanox, thiomethon, thionazin, thuringiensin, tralomethrin, triarathen, triazophos, triazuron, trichlorfon, triflumuron, trimethacarb, vamidothion, XMC, xylylcarb, zetamethrin.

It is preferred to include one or more insecticides chosen among the natural or synthetic pyrethroids e.g. as found above and especially chosen among acrinathrin, cypermethrin, cyfluthrin, cyhalothrin, deltamethrin, fenvalerate and tefluthrin, including any of the previous mentioned compounds in its partially or fully resolved isomeric form. Particularly preferred is acrinathrin.

The substitution of the additional insecticide and/or further addition of other known active compounds, such as herbicides, fungicides, fertilisers or growth regulators, is also possible.

The invention also relates to a process for producing an oil-in-water emulsion formulation as described herein comprising the steps of:

a) preparing an organic phase comprising the phthalate(s), the avermectin(s) and optionally further auxiliaries in the organic phase;

b) preparing an aqueous phase comprising water, the emulsifier system and optionally further hydrophilic auxiliaries;

c) mixing the organic phase and the aqueous phase under agitation to obtain an oil-in-water emulsion.

As the skilled person will easily recognise, the order of addition of the various ingredients used in both the organic and aqueous phase is of minor importance. This also applies to the order of combining the organic phase with the aqueous phase. Some of the optionally auxiliaries may even be added after the mixing of the organic and aqueous phase. One skilled in the art will further recognise that any one of a variety of apparatus may be used to accomplish the mixing steps. Intensive homogenisation is not required. In either of the above steps, heat may be applied to ease the formation of a homogeneous phase.

The invention further relates to the use of oil-in-water emulsion formulations as described herein for the control of pests, said use comprise applying the emulsion, preferably in diluted form (e.g. aqueous diluted form), to the pests or to plants, seeds of plants, soil, surfaces and the like infested with pests or likely to be occupied by pests. While such use is generally aimed at protection of crops against pests (including seeds of crops, e.g. applied as a seed treatment), other applications also form part of the present invention e.g. household uses and veterinary uses including pest control on pets. When used in crop protection, the formulations of the present invention can be used to fight pests such as for example aphids, mites, tics, nematodes, acaricides, roaches, ants and the like.

The formulations according to the invention show bioefficacy comparable to that of conventional EC formulations but at the same time avoids the use of large amounts of hazardous organic solvents and as such are more environmental and user friendly. Further, the formulations significantly reduce the degradation of the avermectin(s) also when exposed to light.

The formulations according to the invention have the following characteristics: A volume-surface mean diameter in the range 1-20 μm, preferably 1-10 μM, no distinct smell, high flash point and are white and free-flowing (200-55000 cP, preferably 200-25000 cP depending on the particular composition of the formulation) following preparation. However, if high pressure homogenization techniques or similar means are employed during the preparation process, oil-in-water formulations with a significant lower volume-surface mean diameter can be prepared as well and are within the scope of the present invention. This may provide an improved efficacy and/or improved homogeneity of the final formulation.

While concentrated formulations are more preferred as commercially available goods, the end consumer uses, as a rule, dilute compositions, as it is well known in the art. Such compositions are part of the present invention.

The invention is illustrated by the following examples, which are provided solely for illustrative purposes and should not be considered limiting:

EXAMPLE 1

1.90 g Abamectin (94.55%) is dissolved in 25 g dimethyl phthalate and a total amount of 6.8 g of preservative, sticker, thickener and co-solvent is added and dissolved. 73.3 g of aqueous phase consisting of a buffer agent, an anionic emulsifier (1.5% w/w of the emulsion) and water is prepared. The emulsification is performed in one of two ways, both resulting in an oil-in-water emulsion of comparable electric conductivity and volume-surface mean diameter of the emulsion droplets. 1) Under vigorous stirring (3000-4000 rpm), the aqueous phase is added to the organic phase and stirring is continued until the volume-surface mean diameter is in the range 1-20 μm. 2) Under vigorous stirring (3000-4000 rpm) the organic phase is added to the aqueous phase and stirring is continued until the volume-surface mean diameter is in the range 1-20 μm. Adjustment of pH and viscosity when relevant are done following the emulsification process. The preparations appear as white non-transparent emulsions.

EXAMPLE 2

Abamectin 18 g/l oil-in-water emulsions containing various oil phases were prepared in accordance with the procedure outlined in example 1 using premium grade of inerts and an optimal combination of emulsifying agents in each emulsion produced. Only the necessary amount of organic solvents was applied in order to keep the Abamectin dissolved in the oil phase. The stirring speed during the emulsion formation was regulated such that the volume-surface mean diameter was in the range 1-20 μm after production. Whereas examples A and B in table 1 are prepared according to the invention, examples C through K are comparative.

EXAMPLE 3

Oil-in-water emulsions of Abamectin as active ingredient and dimethyl phthalate as organic solvent are prepared as described in example 1 at a range of pH values and the stability of the active ingredient in accelerated storage tests at 54° C. and 70° C. for 14 days is determined, see table 2. The composition of the studied emulsion is as follows: 1.68% abamectin, 23.4% dimethyl phthalate, 5.6% co-solvent (Shell fluid 2613), 0.86% preservative, antifoam agent, sticker, thickener and citric acid, 1.5% anionic emulsifier (Soprophor FLK) and water up to 99%. pH adjusted using NaOH.

The stability of the active ingredient in the prepared EW formulation is increased by adjusting pH, resulting in an acceptable level of degradation of less than 2% at pH 7 and pH 8 even for storage at 70° C. for 14 days. For storage at 54° C. the pH need only be adjusted to pH 5 to obtain this improvement in stability.

EXAMPLE 4

Oil-in-water emulsions of Abamectin as active ingredient and diethyl phthalate as organic solvent are prepared as described in example 1 at a range of pH values and the stability of the active ingredient in accelerated storage at 54° C. and 70° C. for 14 days is determined, table 3. The composition of the studied emulsion is as follows: 1.7% Abamectin. 23.4% diethyl phthalate. 5.6% co-solvent (Shell fluid 2613), 0.86% preservative, antifoam agent. sticker. thickener and citric acid, 1.5% anionic emulsifier and water up to 99%. pH adjusted with NaOH

 The stability of the active ingredient in the prepared EW formulation is increased by adjusting pH, resulting in an acceptable level of degradation of less than 3% at pH 6 and above even for storage at 70° C. for 14 days. For storage at 54° C. the pH need only be adjusted to pH 5 to obtain this improvement in stability.

EXAMPLE 5

Oil-in-water emulsions of Abamectin as active ingredient and diisononyl phthalate as organic solvent is prepared as described in example 1 at a range of pH values and the stability of the active ingredient in accelerated storage at 54° C. and 70° C. for 14 days is determined (table 4). The composition of the studied emulsion is as follows (% w/w): 0.70% Abamectin, 43.9% diisononyl phthalate, 5.6% co-solvent (Shell fluid 2613), 0.86% of preservative, antifoam agent, sticker, thickener and citric acid, 1.5% anionic emulsifier and water up to 99%. pH adjusted using NaOH

 The stability of the active ingredient in the prepared EW formulation is acceptable in the pH range 5-7 even for storage at 70° C. for 14 days.

EXAMPLE 6

An Abamectin 1.64% oil-in-water emulsion containing 30.7% diethyl phthalate and an Abamectin 1.78% microemulsion was applied on shaved rat skin mounted in Franz diffusion cells. The microemulsion contained, beside Abamectin, 22% cyclohexanone, 15% tristyrylphenol oxethylated with 20 moles of EO phosphorylated and neutralised with triethanolamine emulsifier, 3% sodium salt of an alkyldiglycol ether-sulfate, and 58% de-mineralised water (prepared according to the process set forth in U.S. Pat. No. 5,227,402—example 11).

According to table 5, less Abamectin permeated through the shaved rat skin from the oil-in-water emulsion than from the microemulsion, p<0.05, student's t-test). On average, 48.4 microgram and 17.8 microgram Abamectin permeated through the rat skin from the microemulsion and the oil-in-water emulsion, respectively. A low Abamectin permeation rate through mammal skin is highly desirable.

Another desirable property is a high flash point of the formulation as it can be handled with less risk. The flash point of the oil-in-water emulsion was determined to be higher than 95° C., for the microemulsion the flash point was 53° C. and the commercial available Abamectin EC formulation determined to be 70° C. (Petrotest, closed cup flash point tester, Pensky-Martens, Germany).

EXAMPLE 7

Comparative

The stability of Abamectin in water at various pH-values and temperatures was determined. 194.4 mg of abamectin was dissolved in 10 ml methanol and 1 ml of the solution transferred to 100 ml of de-mineralised water and a part of this was transferred to a buffer solution. The sample was kept in the dark and the solution analysed using a HPLC. Results are provided in table 6.

EXAMPLE 8

Comparative

The stability of Abamectin in water exposed to light at various pH-values was determined. 194.4 mg of abamectin was dissolved in 10 ml methanol and 1 ml of the solution transferred to 100 ml of demineralised water and a part of this transferred to a buffer solution. The solution was exposed to light (5000-6000 lux) at 25° C. and analysed using a HPLC. Results are provided in table 7.

EXAMPLE 9

Oil-in-water emulsions, which contained either 0.85% Abamectin and 3.55% Acrinathrine, formulation I, or 0.22% Abamectin and 6.87% Acrinathrine, formulation II, were prepared. The manufacturing procedure outlined in example 1 was followed strictly, and the inert ingredients content in the present mixture formulations were as outlined in table 1, example A.

According to an accelerated stability study, the chemical stability of both Acrinathrine and Abamectin was excellent in the present mixture formulations. Results are provided in table 8.

EXAMPLE 10

Abamectin oil-in-water emulsions were prepared applying the manufacturing procedure described in Example 1. A mineral oil, Shell Fluid 2613, or a spreading agent, Lutensol A07, were included in the organic phase and the water phase of the emulsions, respectively.

The efficacy of the formulations was measured on Tetranychus urticae mites applying a greenhouse test. The diluted formulations were sprayed on bean plants in a spray cabinet and mites were transferred to the plants right after the leaf surfaces were dry.

According to the efficacy data in table 9, the inclusion of mineral oil, Shell Fluid 2613, or the spreading agent, Lutensol A07, improved the activity of the Abamectin oil-in-water emulsion.

EXAMPLE 11

Abamectin oil-in-water emulsions were prepared applying the manufacturing procedure outlined in Example 1. Emulsions were prepared by adding the water phase to the organic phase or vice versa. After preparation the pH of the emulsions were adjusted to 5.0 with 1M NaOH, and conductivity measurements were done in order to ensure the finished products were oil-in-water and not water-in-oil emulsions.

The composition of the emulsions is tabulated below, table 10. According to the chemical stability data in table 10, both manufacturing procedures, i.e. adding water phase to oil phase or adding oil phase to water phase, gave formulations having excellent chemical stability. In addition, inclusion of a mineral oil, e.g. Shell fluid 2613, appeared to improve the chemical stability of abamectin in the emulsions.

Biological activity of the formulations against Tetranychus urticae mites was studied as described in example 10. Both the fresh formulations and formulations stored for 14 days at 54° C. had excellent activity against the mites.

EXAMPLE 12

An Abamectin 18 g/l oil-in-water emulsion containing diethyl phthalate as organic solvent was prepared applying the method in example 1 using premium grade of inerts and an emulsifying agent. The stirring speed during the emulsion formation was regulated such that the volume-surface mean diameter was in the range 1-20 μm after production.

The efficacy of the formulations was measured in a greenhouse assay and in a field assay. For the greenhouse assay the diluted formulations were sprayed on bean plants in a spray cabinet and mites were transferred to the plants right after the leaf surfaces were dry. The formulation proved comparable to a conventional emulsifiable concentrate (EC) formulation of abamectin in toxicity towards two spotted spider mites (Tetranycus urticae) on bean plants in a greenhouse assay as indicated by the obtained ED50 values reported in table 11. Against Beet Armyworm (Spodoptera exigua) on tradescantis leaves the EW formulation proved to be even more toxic than the conventional EC formulation in the greenhouse assay.

Field trials were also conducted with the oil-in-water formulation showing that the EW and the commercial EC formulations had comparable efficacies against two spotted spider mites (Tetranychus urticae) on aubergine (Solanum melongena L.), as reported in table 12.

EXAMPLE 13

Oil-in-water emulsions of Abamectin as active ingredient and dimethyl phthalate as organic solvent are prepared as described in example 1 using different co-solvents, pH adjusted to 7 using NaOH. Results are provided in table 13.

EXAMPLE 14

An oil-in-water formulation of Abamectin is prepared according to example 1. The composition of the emulsions is as follows: 1.8% Abamectin, 25.0% dimethyl phthalate, 0.9% preservative, antifoam agent, sticker, thickener and buffer, 6% co-solvent (Shell fluid 2613), 6.6% total of two anionic emulsifiers (Soprophor FLK and LFS) and water up to 99%. A microemulsion (ME) of Abamectin also containing lidocaine is prepared according to European patent no 45655-A2, example 1. For each emulsion 1 ml of emulsion is transferred to 4 crystallisation bowls and left to dry in darkness. Two bowls are exposed to light from a xenon lamp (400 lux) for 10 hours and two bowls are left in darkness also for 10 hours. After exposure the formulation is dissolved in 10 ml ethanol and the remaining amount of Abamectin is determined by HPLC analysis. The experiment is repeated using a commercial 18 g/l EC formulation of Abamectin for comparison. Table 14 shows the stability of both prepared emulsions along with results from the conventional EC formulation of Abamectin. The table indicates that the stability of Abamectin under the exposure of light is greater for the emulsion prepared according to example 1 than for the comparative microemulsion and the commercial EC formulation.

EXAMPLE 15

An oil-in-water emulsion containing Ivermectin or Emamectin benzoate as the active ingredient and diethyl phthalate as organic solvent was prepared applying the method in example 1 using premium grade of inerts and an emulsifying agent. The pH of the emulsion is adjusted to 7 using NaOH and the storage stability of the prepared emulsion is studied using accelerated storage tests at 54° C. and 70° C. for 14 days. The results of the storage tests are given in table 15.

 The efficacy of the formulation containing emamectin benzoate was tested in a greenhouse assay. For the greenhouse assay the diluted formulations were sprayed on bean plants in a spray cabinet and the species tested (mites and thrips respectively) were transferred to the plants after the leaf surfaces had dried. The test on Spodoptera exiqua was conducted as a dip-test where Tradescantia crassifolia leaves are dipped in the test solution, dried and then each leaf is infested with 5 Spodoptera exigua.

EXAMPLE 15

An Abamectin 18 g/l oil-in-water emulsion containing diethyl phthalate as organic solvent was prepared applying the method in example 1 using premium grade of inerts and an emulsifying agent. The stirring speed during the emulsion formation was regulated such that the volume-surface mean diameter was in the range 2-4 μm. Afterwards, the emulsion was treated in a high-pressure (intensive) homogenizer. After the treatment the diameter of the droplets were well below 1 μm.

EXAMPLE 16

An Aversectin-C oil-in-water emulsion containing diethyl phthalate as organic solvent was prepared applying the method in example 1 using premium grade of inerts and an emulsifying agent. The prepared composition has an initial content of 2.08% Aversectin-C, and after storage at 54° C. for 14 days the Avesectin-C content was 2.06%.