足底热刺痛仪主要通过Hargreaves法检测动物缩足潜伏期PWL。

疼痛甩尾和冷热板实验虽是急性疼痛热阈值的经典测量方法这两种实验至今仍然被药理学研究采用。但这两种方法都有一些局限性没有在痛觉过敏的行为反应研究中得到运用。

足底测试代表了一种的实验方法它集合了疼痛过敏测试的优点

· 实验时受试动物无拘束可自由活动；

· 实验数据记录是仪器自动感应完成的无需人为判断和记录；

· 通过聚焦红外光源于动物足底按下开关等待动物缩回受测足爪仪器可自动记录红外光强度和持续时间；

· 红外光源设置了一个特殊的过滤器能够过滤掉可见光谱防止可见光干扰动物影响实验结果；

· 带自检装置反馈电路能够进行自检能有效避免错误的实验环境；

· 实验数据会显示在液晶屏上数据可导入U盘或通过USB数据线导入至电脑。

· 文献引用量超过2000的足底热刺激设备；

· 数据在前板上显示可以通过USB传送到电脑上USB储存设备和软件都包含在标准的配件包里

型号37370

产品特点
· 可自动或手动记录爪缩回时间不需要视觉评分无差错测量精确
· 触摸屏控制所有功能和结果查看
· 配备USB接口可单独工作也可连接电脑使用
· 带数据统计软件可将CSV文件从直接导出到USB
· 爪缩回潜伏期的分辨率为0.1s
· 红外光强度01-99 级间可调
· 可选配红外热辐射校准仪用于校准红外光源
· 6只大鼠或12只小鼠同时进行实验

主机及测试光源

主要参数

· 操作方式按键
· 数据读取液晶屏显示
· 红外光强度01-99 级间可调
· 时间分辨率0.1s
· 红外灯泡Halogen "Bellaphot", Mod. 64607 OSRAM, 8V-50W
· 数据转移闪存
· 电源85-264 VAC, 50-60Hz
· 工作温度15°- 30°C
· 噪音< 70dB
· 校准红外辐射计
· 规格85x40x35 cm
· 鼠笼尺寸20x20x14cm数量3个
· 净重13.0kg

可选配Durham大鼠束缚器配合足底热点仪用于大鼠下颌部三叉神经痛测试。

刺激强度值（红外热辐射值）对照表

（单位mW/cm2）

主要配置清单

可选配红外热辐射测量仪

足底热刺痛仪的部分引用文献

《Science》
1.La Montanara, Paolo, et al. "Cyclin-dependent–like kinase 5 is required for pain signaling in human sensory neurons and mouse models." Science translational medicine 12.551 (2020)eaax4846.doi:10.1126/scitranslmed.aax4846 
IF 19.32
2.Feng, Jiao, et al. "A new painkiller nanomedicine to bypass the blood-brain barrier and the use of morphine." Science advances 5.2 (2019)eaau5148.doi10.1126/sciadv.aau5148 
IF 14.96
3.Hsiao, Hung-Tsung, et al. "The analgesic effect of propofol associated with the inhibition of hypoxia inducible factor and inflammasome in complex regional pain syndrome." Journal of medical science 26 (2019)1-11. doi:10.1186/s12929-019-0576-z 
IF 12.77
4.Zhou, Luming, et al. "Reversible CD8 T cell–neuron cross-talk causes aging-dependent neuronal regenerative decline." Science 376.6594 (2022)eabd5926. doi10.1126/science.abd5926
IF 63.71

《Nature》

5.Oswald, Manfred J., et al. "Cholinergic basal forebrain nucleus of Meynert regulates chronic pain-like behavior via modulation of the prelimbic cortex." Nature Communications 13.1 (2022)5014.doi: 
IF 17.69
6.Landra-Willm, Arnaud, et al. "A photoswitchable inhibitor of TREK channels controls pain in wild-type intact freely moving animals." Nature Communications 14.1 (2023)1160.doi: 
IF 17.69
7.Nees, Timo A., et al. "Role of TMEM100 in mechanically insensitive nociceptor un-silencing." Nature Communications 14.1 (2023)1899.
doi10.1038/s41467-023-36806-4
IF 17.69
8.Zhang, Qiaosheng, et al. "A prototype closed-loop brain–machine interface for the study and treatment of pain." Nature Biomedical Engineering (2021)1-13. doi10.1038/s41551-021-00736-7
IF 29.23
9.Zhang, Su-Bo, et al. "CircAnks1a in the spinal cord regulates hypersensitivity in a rodent model of neuropathic pain." Nature communications 10.1 (2019)4119.doi:1
10.IF17.69  
11.Jiang, Wenhao, et al. "PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis." Bone research 10.1 (2022)27. doi:10.1038/s41413-022-00201-4
13.36
12.Bao, Yi-Ni, et al. "The dopamine D1–D2DR complex in the rat spinal cord promotes neuropathic pain by increasing neuronal excitability after chronic constriction injury." Experimental & Molecular Medicine 53.2 (2021)235-249.doi:10.1038/s12276-021-00563-5
IF 12.15
13.Takeda, Ikuko, et al. "Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour." Nature communications 13.1 (2022)4100.doi10.1038/s41467-022-31773-8
IF 17.69
14.Liang, Hai-Ying et al. “nNOS-expressing neurons in the vmPFC transform pPVT-derived chronic pain signals into anxiety behaviors." Nature communications vol. 11,1 2501. 19 May. 2020, doi:10.1038/s41467-020-16198-5 doi:10.1038/s41467-020-16198-5
IF 17.69
15.Zhou, Hang, et al. "A sleep-active basalocortical pathway crucial for generation and maintenance of chronic pain." Nature Neuroscience (2023)1-12. doi10.1038/s41593-022-01250-y
IF 28.77
16.Wang, Yan et al. “TRPV1 SUMOylation regulates nociceptive signaling in models of inflammatory pain." Nature communications vol. 9,1 1529. 18 Apr. 2018, doi10.1038/s41467-018-03974-7 
IF 17.69
17.Iwasaki, Mai, et al. "An analgesic pathway from parvocellular oxytocin neurons to the periaqueductal gray in rats." Nature Communications 14.1 (2023)1066. doi:10.1038/s41467-023-36641-7
IF 17.69

《Cell》
18.Zhang, Fang-Xiong et al. “BK Potassium Channels Suppress Cavα2δ Subunit Function to Reduce Inflammatory and Neuropathic Pain." Cell reports vol. 22,8 (2018)1956-1964. doi:10.1016/j.celrep.2018.01.073 
IF 10.00
19.Gui, Xianwei et al. “Botulinum toxin type A promotes microglial M2 polarization and suppresses chronic constriction injury-induced neuropathic pain through the P2X7 receptor." Cell & science vol. 10 45. 23 Mar. 2020, doi:10.1186/s13578-020-00405-3

方法学文献

K.M. Hargreaves, R. Dubner, F. Brown, C. Flores and J. Joris"A New and Sensitive Method for Measuring Thermal Nociception in Cutaneous Hy-peralgesia" Pain 3277-88, 1988

D.C. Yeomans & H.K. Proudfit"Characterization of the Foot Withdrawal Response to Noxious Radiant Heat in the Rat" Pain 5985-97, 1994